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  • Vitamin B12 deficiency is estimated to occur in up to 20% of the elderly population
  • 70% of Vitamin B12 in the blood is biologically inactive. The Active-B12 test measures only the bio-active portion.
  • A low Active-B12 level is proposed to be the earliest marker of Vitamin B12 deficiency


The ACTIVE-B12 (Holotranscobalamin) assay from Axis-Shield is a new and innovative way of assessing Vitamin B12 levels and offers improved accuracy, sensitivity and specificity over the current out-dated front-line tests.



We aim to continue to present new findings into the clinical usefulness of measuring Active-B12 (Holotranscobalamin). We aim to make the test as widely available as possible, in the routine laboratory setting and for research, with the goal of improving the lives of patients suffering from Vitamin B12 deficiency.


Keep checking this site for new research findings in this area that should make the task of diagnosing vitamin B12 deficiency easier for the laboratorian, the doctor and the patient..


Axis-Shield will develop the Active-B12 test for Siemens Healthcare allowing more laboratories worldwide to perform the test.


A new publication has demonstrated that five different Total B12 assays gave normal B12 results in a patient with confirmed pernicious anaemia. To access the paper click here.


An experts meeting at a recent conference produced a Consensus Statement on the improved utility of the Active-B12 assay over Total B12. To see the statement click here.

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